簡介：內(nèi)蒙古農(nóng)業(yè)大學(xué)碩士學(xué)位論文奶牛環(huán)境性乳腺炎滅活疫苗的研制及效果評價姓名趙建榮申請學(xué)位級別碩士專業(yè)預(yù)防獸醫(yī)學(xué)指導(dǎo)教師郝永清20090501DEVELOPMENTANDEVALUATIONOFEFFECTIVENESSOFINACTIVATEDVACCINEOFBOVINEENVIRONMENTALMASTITISBSTRACT’MASTIFFSISINFLAMMATIONOFMAMMARYGLANDBECAUSEOFVARIOUSCAUSATIVEORGANISMMASTIFFSBECOMESMAINRESOILLGEOFDELETERIOUSBACTERIUMOFMILKANDMILKPRODUCTITALSOBECOMESODMMON謝也DISEASEOFMALLANDANIMALESPECIALLY，ETIOLOGICALFACTORSOFENVIRONMENTALMASTIFFSALEVERYCOMPLICATINGANDSPECIESOFCAUSATIVEORGANISMARERELATIVELYMUCHMORETHEREFORE，THEEXPERIMENTCALRYSOUTANALYSESOFIMMUNOGENICITYOFMAINCAUSATIVEORGANISMOFENVIRONMENTALMASTITISTHATHASBEENALREADYSEPARATEDANDIDENTIFIEDANDPREPARESVACCINEIHOPEEXPERIMENTWILLPROVIDENEWMETHODANDROUTETOPREVENTANDCBREENVIRONMENTALMASTITISININNERMONGOLIATOSELECTSTRAINSOFGOODIMMUNOGENICITYANDEXTENSIVEANTIGENSPECTRUM2SESCHERICHIACOLISAND2SKLEBSIELLASAREPROCESSED、現(xiàn)出SDSPAGEANDWESTERNBLOTTHERESULTSSHOWTHATTHEPROTEINBANDSOFALANDKLAREMOREFORTISTHANA2ANDK2TOPROGRESSTHOROUGHLYIMMUNOGENICITYANALYSIS，ELISAISESTABLISHEDTODETECTTHEANTIBODYTITERSANDTHECROSSPROTECTIVEANTIBODYINBLOODSERBINOFRABBITAFTERIMMUNIZATIONAMANDKLOFTYPICALANTIGENSPECTRUMANDALLROUNDPROTECTIVEANTIGENSALESUITABLETOBECOMESTRAINSOFPREPARINGVACCINETHEBACTERIALSUSPENSIONOFALANDKLISUNACTIVATED謝NLFORMALDEHYDESANDPROGRESSEDGERMFREETESTALHYDROGELADJUVANTISSELECTEDTOMAKEINACTIVATEDVACCINEAGAINSTENVIRONMENTALMASTITISANDTHEVACCINEISTESTED、析THSAFETYCOLLECTINGBLOODSERUMOFRABBITSAFTERTHREETIMESIMMUNIZATION、析也THEVACCINEISTESTEDSPECIFICANTIBODYBYMETHODOFELISATHERESULTSSHOWTHEVACCINEAGAINSTENVIRONMENTALMASTITISOFCOWSBYMIXING她也ALHYDROGELISSAFENEVACCINEALSOCANSUSCITATERABBITSTOPRODUCEHI曲SPECIFICANTIBODYWITHTHEMOUSEMODEL，CHALLENGETESTISPROCESSEDAFTERTHREETIMESIMMUNIZATIONWI吐LTHEVACCINETHERESULTSSHOWEFFECTIVEPROTECTIONAGAINSTESCHERICHIACOLIANDKLEBSIELLAOFHOMOLOGOUSANDVARIOUSSEROLOGICALTYPESCOULDBEGAINEDTOESTIMATEEFFECTOFPREVENTANDCU他BOVINEENVIRONMENTALMASTITISBYINOCULATINGINACTIVATEVACCINETOCARRYOUTEXPERIMENTOFINOCULATINGVACCINEFORCOWSINGRAZINGLANDOFINNERMONGOLIAAGRICULTURALUNIVERSITYEXPERIMENTESTABLISHESELISATOTESTSPECIFICANTIBODYINBLOOD翻胃瑚ANDNLI墩翻器瑚FOREVALUATINGEFFECTOFIMMUNITY，TESTINGSPECIFICANTIBODYINBLOOD∞NLMANDIILILKSERUMAFTERTHREETIMESIMMUNIZATION謝ⅡLTHEVACCINE，THERESULTSSHOWTHATSPECIFICANTIBODYOFIMMUNITYGROUPINBLOODSERUMANDIILI墩

簡介：CLASSIFIEDINDEXCONFIDENTIALYES／NONOCODE10224NO120910799DISSERTATIONFORTHEMASTERDEGREERESEARCHONTHEEFFECTOFSYKONDAIRYCOWMAMMARYEPITHELIALCELLPROLIFERATIONCANDIDATELINLINSUPERVISORPROFESSORLINYEDEGREECATEGORYMASTEROFSCIENCECOLLEGECOLLEGEOFLIFESCIENCEFIRSTLEVELDISCIPLINEBIOLOGYSECONDLEVELDISCIPLINEBIOCHEMISTRYANDMOLECULARBIOLOGYHARBINCHINAJUNE2015東北農(nóng)業(yè)大學(xué)理學(xué)碩士學(xué)位論文245提取質(zhì)粒24246帶粘性末端質(zhì)粒的制備24247連接與轉(zhuǎn)化25248AKTL啟動子的PGL3真核表達載體的鑒定。25249去內(nèi)毒素質(zhì)粒的提取252410去內(nèi)毒素質(zhì)粒轉(zhuǎn)染奶牛乳腺上皮細胞252411熒光素酶活性檢測2625數(shù)據(jù)處理與統(tǒng)計分析263結(jié)果與分析。2731奶牛乳腺組織中SYK的表達與定位27311奶牛乳腺組織中SYK的表達含量變化27312奶牛乳腺組織中SYK的表達部位2832SYK基因?qū)δ膛Ｈ橄偕掀ぜ毎鲋车挠绊?9321奶牛乳腺上皮細胞原代培養(yǎng)29322奶牛乳腺上皮細胞的鑒定30323SYK基因沉寂對細胞增殖的影響31324SYK基因過表達對細胞增殖的影響3333SYK基因調(diào)節(jié)奶牛乳腺上皮細胞增殖的機制一37331AKTL基因啟動子區(qū)PCR擴增一37332PGL3AKTL真核表達載體構(gòu)建結(jié)果37333熒光素酶活性檢測結(jié)果374討侖3941奶牛乳腺組織中SYK基因的表達與定位分析3942SYK基因?qū)δ膛Ｈ橄偕掀ぜ毎鲋车挠绊懸?943SYK基因調(diào)節(jié)乳腺上皮細胞增殖的機制405結(jié)論42致射43參考文獻44攻讀碩士期間發(fā)表的學(xué)術(shù)論文49II

簡介：ISOLATIONANDCULTUREANDIDENTIFICATIONOFCOWMAMMARYSTEMCTHESISSUBMITTEDTOQINGDAOAGRICULTURALUNIVERSITYINFULFILLMENTOFTHEREQUIREMENTFORTHEDEGREEOFMASTEROFAGRONOMYLIUCHANGJUCOLLEGEOFANIMALSCIENCEANDVETERINARYMEDICANESUPERVISORPROFDONGYAJUANQINGDAOCHINAJUNE2009112試劑藥品17113試驗儀器1712試驗方法18121奶牛MASCS的分離及原代培養(yǎng)18122奶牛MASCS的傳代培養(yǎng)18123奶牛MASCS生長曲線的繪制。19124奶牛MASCS的瑞氏吉姆薩RSGMS染色一19125奶牛MASCS的凍存與復(fù)蘇1913結(jié)果與分析20131奶牛MASCS的分離及原代培養(yǎng)～20132奶牛MASCS的傳代培養(yǎng)20133奶牛MASCS生長曲線的繪制_21134奶牛MASCS瑞氏吉姆薩RSGMS染色2L135奶牛MASCS的凍存與復(fù)蘇2214討論222不同月齡奶牛MASCS的生物學(xué)特性比較2621試驗材料和方法26211試驗材料26212試劑藥品26213試驗儀器26214試驗分組26?！?2試驗方法27221不同月齡奶牛MASCS的分離及原代培養(yǎng)27222不同月齡奶牛MASCS的傳代培養(yǎng)27223不同月齡MASCS生長曲線的繪制一27224不同月齡奶牛MASCS貼壁率的測定2723結(jié)果與分析28231不同月齡奶牛MASCS原代培養(yǎng)形態(tài)學(xué)觀察結(jié)果28232不同月齡奶牛MASCS傳代培養(yǎng)形態(tài)學(xué)觀察結(jié)果29

簡介：STUDIESONGENETICVARIATIONOFHSFLGENEANDITSASSOCIATL0NⅥ礓THGLANDHEALTHINCATTLEBYⅪAPENGSUPERVISORPROFWANGGENLINATHESISSUBMITTEDTONANJINGAGRICULTURALUNIVERSITYINPARTIALFUWLLLMENTOFTHEREQUIREMENTSFORMASTERDEGREEOFAGRONOMYTHECOLLEGEOFANIMALSCIENCEANDTECHNOLOGYNANJINGAGRICULTURALUNIVERSITYNANJING，210095，PRCHINACOMPLETEDINAPRIL，2012COMMENCEMENTINMAY，2012㈣∞刪5Ⅲ刪1刪6刪3㈣2刪Y本研究由“十二五’’國家科技計劃課題編號2011BAD28802，2012BADL2810和江蘇省農(nóng)業(yè)三新工程課題編號SX2011189資助SUPPORTEDBYNATIONALSUPPORTINGPROJECTSFORSCIENCEANDTECHNIQUES州O2011BAD28802，2012BADL2810ANDPROVINCIALPROJECTFORAGRICULTURALTECHNIQUES科OSX2011189

簡介：分類號華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文大豆異黃酮與玉米赤霉烯酮對乳腺癌細胞系MCF一7聯(lián)合作用的的研究RESEARCHOFINTERACTIONOFIS0ANDZEAONBREASTCANCER研究生指導(dǎo)教師MCF7CELLS馬青山齊德生教授專業(yè)動物營養(yǎng)與飼料科學(xué)研究方向飼料毒物與抗?fàn)I養(yǎng)因子獲得學(xué)位名稱農(nóng)學(xué)碩士獲得學(xué)位時間華中農(nóng)業(yè)大學(xué)動物科技學(xué)院中國武漢華中農(nóng)業(yè)大學(xué)學(xué)位論文獨創(chuàng)性聲明及使用授權(quán)書學(xué)位論文是如需保密，解密時間二0／O年占月／P日是否保密獨創(chuàng)性聲明本人聲明所呈交的論文是我個人在導(dǎo)師指導(dǎo)下進行的研究工作及取得的研究成果。盡我所知，除了文中特別加以標(biāo)注和致謝的地方外，論文中不包含其他人已經(jīng)發(fā)表或撰寫過的研究成果，也不包含為獲得華中農(nóng)業(yè)大學(xué)或其他教育機構(gòu)的學(xué)位或證書而使用過的材料，指導(dǎo)教師對此進行了審定與我一同工作的同志對本研究所做的任何貢獻均已在論文中做了明確的說明，并表示了謝意。研究生簽名馬者以時間如。7年多月／日學(xué)位論文使用授權(quán)書學(xué)位敝儲張多荔厶翩張一丟岳譬一獅肌1幽／FEJ拼飆7㈣乃注請將本表直接裝訂在學(xué)位論文的扉頁和目錄之間

簡介：東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文中藥乳頭灌注劑治療臨床型奶牛乳腺炎的研究姓名張振國申請學(xué)位級別碩士專業(yè)臨床獸醫(yī)學(xué)指導(dǎo)教師侯振中20090620ABSTRACTⅢSTUDIESONTRADITIONALCHINESEMEDICINEPERFUSIONSTOTREATCLINICALMASTITISINDAIRYCOWSABSTRACTCOWMASTITISHASGREATHARMTODAIRYCOWBREEDINGINDUSTRY．ITREDUCESMILKQUANTITYANDQUALITY,WHICHMAKETHEGREATECONOMICLOSSESTHEREFORE,ITISINCREASINGLYIMPORTANTHOWTOEFFECTIVELYPREVENTANDCURECOWMASTITISINTHISEXPERIMENT,THROUGHANTIBACTERIALTESTANDORTHOGONALTEST,SELECTEDSEVERALCHINESEHERBALMEDICINEWHICHWERESENSITIVETOCLINICALMASTITISPATHOGENICBACTERIAISOLATEDFROMCLINICALMASTITISCOWINHARBINTHEOPTIMALTRADITIONALCHINESEMEDICINECOMPOSITIONWASDETERMINEDBASEONTHERATIONALEOFTHETRADITIONALCHINESEMEDICINEPHARMACEUTICSCLINICALPHARMACODYNAMICSTESTANDSAFETESTOFTHENIPPLEPERFUSIONOFTHETRADITIONALCHINESEMEDICINEWASMADESIMULTANEOUSLY,WEDETECTEDTHECONTENTSOFSOD、MDA、NO、GSHPX、LDH、TAOCINWHEYBEFOREANDAFTERUSINGMEDICINEWHICHCOULDINDIRECTLYREFLECTTHESTATEOFIMMUNESYSTEMANDMAMMARYGLANDREPAIRTIONOFDAIRYCOWTHESTUDIESSHOWEDTHATMAINPATHOGENICBACTERIAOFCLINICALMASTITISWASSTREPTOCOCCUSMASTITIDIS,STAPHYLOCOCCUSAUREUSANDESCHERICHIACOLISEVENCHINESEMEDICINALHERBDANDELION,HEARTLEAFHOUTTUYNIAHERB,LIGUSTICUM,ANDSOONMADEUPCOMPOSITIONTHROUGHANTIBACTERIALTEST,ORTHOGONALTESTANDTHETHEORYOFVETERINARIANTHEPERFUSIONOFTHETRADITIONALCHINESEMEDICINEWASSAFETYTHROUGHSAFETESTTHEPERFUSIONHADGOODTHERAPEUTICACTIONONCLINICALMASTITISOFDAIRYCOWEFFECTIVERATE100,HEALINGRATE9091THECONTENTOFSOD,GSHPX,TAOCINWHEYAFTERUSINGDRUGWASOBVIOUSLYHIGHERTHANBEFOREP005,ANDTHECONTENTOFMDAANDNOINWHEYWASSIGNIFICANTLYLOWERTHANBEFOREP005,THECONTENTOFLDHDIDNOTCHANGESIGNIFICANTLYP＞005THESTUDIESSHOWEDTHATTHEPERFUSIONOFTHETRADITIONALCHINESEMEDICINESWASASAFEANDGOODEFFICACYDRUGTOCLINICALMASTITISWHICHCOULDPROVIDESOMEBENEFICIALATTEMPTFORPURFUSIONOFTHETRADITIONALCHINESEMEDICINETOTHEAPPLICATIONANDDEVELOPMENTOFSTOCKFARMINGKEYWORDSCOWS,MASTITIS,THETRADITIONALCHINESEMEDICINE,BREASTPERFUSIONS,ANTIOXIDANTSYSTEMCANDIDATEZHANGZHENGUOSPECIALITYCLINICALVETERINARYMEDICINESUPERVISORPROFHOUZHENZHONG

簡介：977407HJ§掛L一FOJ西弛農(nóng)林釬捉大學(xué)2006屆攻讀碩士學(xué)位研究生學(xué)位畢業(yè)論文催乳素和TPA乳腺共表達載體構(gòu)建和細胞表達研究學(xué)科專業(yè)L哂匪置匡堂研究方向塾塑題照王猩研究生藍』生藍指導(dǎo)教帥堂插教授完成時間墊盟圭理且一中國陜目輛凌CONSTRUCTIONOFMAⅣ卟嗄ARYGLANDDUAL．EXPRESSIONVECTORCONTAININGPROLACTINANDTPAGENEANDITSEXPRESSIONINCELLSABSTRACTINTHISRESEARCH，PROLACTINGENEWASCLONEDFROMGUANGZHONGGOATANDTHEMAMMARYGLANDDUALEXPRESSIONVECTORPPTWASCONSTRUCTEDWHICHCONTAINEDPROLACTINANDTPAGENE．THENPPTWASTRANSFECTEDINTOMICEMAMMARYGLANDCANCERCELLSCULTURED／NVITROANDTHEEXPRESSIONCELLSTRAINSWEREABTAINED．HIGHLEVELEXPRESSIONVECTORWASPROVIDEDFURTHEMAMMARYGLANDBIOREACTORBYNUCLEARTRANSFER．THISRESEARCHCANHELPTOSOLVEPROBLEMONHOWTOINCREASETHEEXPRESSIONOFFOREIGNPROTEIN．THERESULTSOFRESEARCHISASFOLLOWING1．GOATPROLACTINEDNAWASCLONEDFROMGUANZHONGMILKGOATANTERIORPITUITARYRNAWITHRTPCR，WHICHWAS760BPANDCOMPRISEDINTACTCODINGREGIONS．SEQUENCEANALYSISDEMONSTRATEDTHATITSHOMOLOGYWITHTHERELATIVEREGIONOFPROLACTINONGENBANKWAS99％．ANDMUTATIONSCOULDNOTALTERPROTEIN’SSTRUCTUREANDFUNCTION．2．BYTHESTRATEGYOFDIRECTIONALCLONING，PROLACTINGENEWASINSERTEDTOMAMMARYGLANDEXPRESSIONVECTORPEBTANDMAMMARYGLANDDUALEXPRESSIONVECTORPPTWASCONSTRUCTEDWHICHCONTAINEDPROLACTINANDTPAGENE，WHICHINCLUDEDRESISTANTGENEANDEGFPREPORTGENE．ANDEGFPANDAIMEDGENEEXPRESSFUSIONPROTEIN．3．THEVECTORPPTWASTRANSFECTEDINTOMICEBOVINEEARFIBROBLASTMAMMARYGLANDCANCERCELLSCULTUREDINVITROUSINGLIPOSOME．AFTERG418FASTNESSSCREENING，THEPOSITIVECELLSLOWLEVELEXPRESSEDSTABLY．PCRIDENTIFICATIONDEMONSTRATEDTHATTHEVECTORHADINTEGRATEDINTOMICEMAMMARYGLANDCANERCELLULARCHROMOSOMES．THECELLSWHICHTRANSFECTEDPPTEXPRESSEDMOREEGFPPROTEINTHANCELLSTRANSFECTEDPEBTKEYWORDSPROLACTIN，MAMMARYGLANDDUALEXPRESSIONVECTOR，TRANSFECTION，EGFP

簡介：東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文HMMEPDT致犬乳腺腫瘤細胞凋亡相關(guān)因子的研究姓名馬興乾申請學(xué)位級別碩士專業(yè)臨床獸醫(yī)學(xué)指導(dǎo)教師劉云20090620東北農(nóng)業(yè)大學(xué)農(nóng)學(xué)碩士學(xué)位論文IISTUDIESOFRELATEDAPOPTOSISFACTSONCANINEBREASTTUMCELLWITHHMMEPDTABSTRACTCANINEBREASTTUMOCCUREDFREQUENTLYINTHEVETERINARYCLINICSATPRESENTBREASTCANCERISALWAYSTREATEDBYSURGERYLONGTERMSURVIVALRATEHASN’TBEENIMPROVEDWITHTHEDEVELOPMENTOFTHEMODERNTECHNOLOGYPHOTODYNAMICTHERAPYPDTBECOMESANEWCHOICETOTREATMALIGNANTTUMBYPHOTOSENSITIZERLIGHTOXYGENMOLECULEPHOTOSENSITIZERCANBEABSBEDELECTIVELYBYPATHOLOGICALTARGETTISSUEACTIVEOXYGENWILLBEPRODUCEDIFITISSTIMULATEDBYLIGHTWITHSUITABLEWAVELENGTHTREATEDBYENERGYTRANSFMITINJURESTHETARGETTISSUESOITISATUMTHERAPYMETHODWITHGOODDEVELOPINGPROSPECTWHICHCANINDUCEAPOPTOSISNECROSISHEMATOPPHYRINMONOMETHYLETHERHMMEISANEWPHOTOSENSITIZERHASBEENPROVEDITSHIGHPERFMANCEINFOUNDATIONALCLINICALEXPERIMENTSBUTNOWRESEARCHESABOUTTHEEFFECTWITHHMMEPDTONCANINEBREASTTUMCELLARESTILLLESSATHOMEABROADTHENEWHMMETHEHENELASEROFWAVELENGHOF6328NMIRRADIATETHEBREASTTUMCELLLINEWASUSEDINTHISSTUDYTOINVESTIGATEMECHANISMOFPDTONCANINEBREASTCANCERCHMMCELLINDERTOPROVIDEABASISFTHETREATMENTOFCANINEBREASTCANCERDISEASECELLLINESWERECOLLECTEDISOLATEDFROMTHECLINICALMETASTATICBREASTCANCERCULTUREDNMALLYCELLSDURINGEXPONENTIALPHASEOFGROWTHWEREDIVIDEDINTOGROUPSBLANKCONTROLLASERGROUPPHOTOSENSITIZERGROUPCOMBINEDGROUPLASERILLUMINATIONPHOTOSENSITIZERHMMEPDTEXPERIMENTWASUSEDFCANINEBREASTCANCERCHMMCELLSTHEOD490WASDETECTEDWITHMTTALSOEFFECTOFPDTONTHECULTUREOFCANINEBREASTTUMCELLSINVITROWASDETECTEDINHIBITIONCURVEWASDRAWNTHENCELLSRATEWEREDETECTEDWITHTRYPANBLUEEXCLUSIONTESTAPOPTOSISWASDETECTEDWITHHOCHEST33258STAINNINGELECTROPHETICTECHNIQUECELLCYCLEWASDETECTEDWITHFLOWCYTOMETRYCONTENTOFMDANOACTIVITYOFSODGSHPXWEREDETECTEDWITHCHROMATOMETRYCONTENTOFIL2IL6WASDETECTEDWITHRADIOMMUNOASSAYTHEWEREASFOLLOWSBLANKCONTROLPHOTOSENSITIZERGROUPHMME20GMLLASERGROUP28JCM2HADNOOBVIOUSEFFECTONTHECELLMULTIPLICATIONBUTTHEHMMEPDTHMME20GML28JCM2GROUPCELLMULTIPLICATIONWASOBVIOUSLYINHIBITEDSOITPROVEDCANINEBREASTCANCERCHMMCELLSINVITROWEREINHIBITEDWITHHMMEPDTWITHINCERTAINPREMISESPHOTODYNAMICEFFECTPHOTOSENSITIZERDENSITYILLUMINATIONDOSESHADRELATIONSAFTERHMMEPDTTHEREWASOBVIOUSCELLULARAPOPTOSISAPOPTOSISWASMEOBVIOUSASTIMEPROLONGINGATDIFFERENTTIMEINCELLCYCLERATEINCREASEOFG0G1PHRASEHADSIGNIFICANTDIFFERENCEAT12H24H48HCOMPAREDWITHTHECONTROLGROUPP001HOWEVERRATEDECREASEOFSPHRASE

簡介：分類S8167UDC636學(xué)校代碼10129學(xué)號2009201031瘦素對奶牛乳腺上皮細胞乳蛋白合成的調(diào)控作用研究EFFECTSOFLEPTINONMILKPROTEINSYNTHESESINBOVINEMAMMARYEPITHELIALCELLS申請人王春艷學(xué)科門類農(nóng)學(xué)學(xué)科專業(yè)動物營養(yǎng)與飼料科學(xué)研究方向反芻動物營養(yǎng)調(diào)控理論與技術(shù)指導(dǎo)教師高民研究員論文提交日期二〇一二年五月EFFECTSOFLEPTINONMILKPROTEINSYNTHESESINBOVINEMAMMARYEPITHELIALCELLSABSTRACTINTHISEXPERIMENT,EPITHELIALCELLSCULTURETECHNIQUESANDCELLBIOLOGYANDMOLECULARBIOLOGYTECHNIQUESWEREUSEDTOSTUDYLEPTINONMILKPROTEINSYNTHESESOFBOVINEMAMMARYEPITHELIALCELLSTHEFINDINGSINCLUDETHREEPARTSASFOLL0W1MAMMARYEPITHELIALCELLSWEREOBTAINEDFROMTHELACTATINGHOLSTEINCOWSBYENZYMEDIGESTIONANDCULTUREDSUCCESSFULLYINVITROTHECELLSAFTERPURIFIEDBYTRYPSINWERESTOREDINLIQUIDNITROGEN,ANDDETECTEDTHESURVIVALRATEOFRECOVEREDCELLSIMMUNOFLUORESCENCESTAININGSKELETONPROTEINKERATININDICATEDTHATTHECELLSWECULTUREDREALLYWEREBOVINEMAMMARYEPITHELIALCELLSOBSERVINGFROMINVERTEDMICROSCOPE,CELLSWERECOBBLESTONEAPPEARANCEANDADHERENCETHERESULTSSHOWEDTHATTHECELLSWEHARVESTEDCANBEUSEDTOTHEFOLLOWINGSTUDIES2ONTHEBASICOFCULTURESYSTEMOFMAMMARYEPITHELIALCELLSINVITRO,THETREATMENTSOFCELLSINCLUDED4DOESOFLEPTIN0,10,100AND1000NG/MLWITH1ΜG/MLORWITHOUT0PROLACTINPRL,AND0NG/MLLEPTINWASTHECONTROLGROUPTHERESULTSOFDETECTINGCELLPROLIFERATIONBYMTTWERETHATLEPTINPROMOTEDCELLPROLIFERATIONASTIMEDEPENDENCEANDATTHESAMETIME,10AND100NG/MLLEPTINUNCOMMONLYPROMOTEDCELLPROLIFERATION,BUT1000NG/MLLEPTINSIGNIFICANTLYINHIBITEDPROLIFERATIONTHEEFFECTWITHOUTPRLISNOTNOTABLE3THEEXPERIMENTDESIGNOFTHISPARTWASSAMETOPART2THEEXPRESSIONOFGENESWASFROMRTPCREXPERIMENTSANDPROTEINSECRETIONWASANALYZEDBYELISAKITTHERESULTSSHOWEDTHAT1THEEXPRESSIONOFMAINMILKPROTEINGENESWASENHANCEDBYLEPTININTHEPRESENCEOFPRL,ANDTHEREWEREDIFFERENTEFFECTSWITHDIFFERENTLEPTINTREATMENTS21000NG/MLLEPTININHIBITEDPRLRECEPTORPRLREXPRESSIONSIGNIFICANTLYWITHPRLANDPRLOBVIOUSLYINDUCEDPRLREXPRESSION3THEEXPRESSIONOFJAK2WASSAMETOTHATOFMILKPROTEIN,BUTTHEREWASNOEFFECTOFLEPTINONTHEEXPRESSIONOFSTAT54NOEFFECTOFLEPTINONTHESECRETIONOFΑCASIENPROTEIN,10NG/MLLEPTINHASTENEDTHESECRETIONOFΒCASIENPROTEINAND1000NG/MLLEPTININHIBITEDTHATSIGNIFICANTLYWITHPRLTHESTUDYISAUSEFULEXPLORATIONONMOLECULEINTERCOMBETWEENMAMMARYFATPADANDEPITHELIALCELLSANDINORDERTOLAYATHEORETICALFOUNDATIONFORFUTUREPRACTICALAPPLICATIONOFANIMALHUSBANDRY

簡介：西北農(nóng)林科技大學(xué)碩士學(xué)位論文豬瘟病毒E2基因乳腺特異表達載體的構(gòu)建與細胞表達的研究姓名顧玲申請學(xué)位級別碩士專業(yè)臨床獸醫(yī)學(xué)指導(dǎo)教師張涌20040601豬瘟病毒E基因乳腺特異表達載體的構(gòu)建與細胞表達的研究ABSTRACTCLASSICALSWINEFEVERISAHARMFULDISEASETOSWINEHUSBANDRYCLASSICALSWINEFEVERVIRUSCSFVISITSCAUSEANDITSMAINPROTECTIVEANTIGENISGLYCOPROTEINE2ANIMALSCANPREVENTFROMDEADLYVIRUSSATTACKIFTHEYVACCINATEPROTEINE2COMINGFROMEUKARYOTICEXPRESSIONSYSTEMAHEADLYPRODUCINGBIOACTIVEPROTEINSTHROUGHMAMMARYGLANDBIOREACTORISAHOTFOCUSINTHEFIELDOFBIOLOGYANDTHEKEYQUESTIONISTHECONSTRUCTIONOFEXPRESSIONVECTORINMAMMARYGLANDBETALACTOGLOBULINBLGISTHEMAINPROTEMMONALRUMINANTMILKFORTHEPURPOSEOFCONSTRUCTINGMAMMARYGLANDSPECIFICEXPRESSIVECTORWEUSETHEUPDREAMREGULATIONSEQUENCEOFBOVINEBLGTOREGULATECSFVE2GENETOEXPRESSINMAMMARYEPITHELIALCELLSOVERIFYTHEEXPRESSIONEFICIENCYOFFOREIGNGENEANDRATIONALITYOFRPOSITIVECLONECELLSARESELECTEDANDPURIFIEDBYTHEEXPRESSIONANANEOANDREPORTGENEEGFPSOTHECELLSCANBEUSEDASSOURCGENEASPROMOTERINVITROINORDERTEXPRESSIONALVECTOANTIBIOTICSGENEKTOCREATETRANSGENIOFESCANIMALSBYNUCLEARTRANSFERTECHNIQUESTHERESULTSOFRESEARCHAREASFOLLOWINGBOVINEBLGGENEUPDREAMREGULATIONSEQUENCEISCLONEDBYMETHODOFPCRFROMCOWBLOODITCONSISTSOF5FLANKSEQUENCEABOUT645BPANDTHESIGNALPEPTIDESEQUENCEINFIRSTEXONABOUT92BPAFTERPCRPRODUCTISRECOVEREDANDPURIFIEDITISCLONEDINTOTSITEOFPNM18TVECTORTHENITISSEQUENCEDANDCOMPAREDWITHOTHERBOVINEBLGGENEINGENBANKTHERESULTSINDICATETHEHOMOLOGYAREALLABOVE9000ATLASTTHESEQUENCEISANALYZEDBYCOMPUTERWEFINDTHATMANYFACTORSORPROTEINBINDINGSITESANDRESPONSEELEMENTSUCHASMAMMARYSPECIFICBINDINGFACTORMSBFNUCLEARFACTOR1NF1RETINOICACIDRESPONSEELEMENTRAREANDTPARESPONSEELEMENTTREAREINCLUDEINITITSUGGESTESTHECLONEDREGULATORYELEMENTCOULDDIRECTFOREIGNGENESTOEXPRESSSPECIFICALLYINMAMMARYGLANDEPITHELIACELLSANDITCOULDBEUSEDTOCONSTRUCTMAMMARYGLANDSPECIFICEXPRESSIONALVECTORBLGREGULATIONSEQUENCEANDCSFVE2GENEARELINKEDBYMETHODOFRECOMBINANTPCRANDTHENTHEYAREINSERTEDINTOPMD18TVECTORTHEPOSITIVERECOMBINANTPLASMIDISMARKEDASPBETHECOMPOSEDFRAGMENTBEISOBTAINEDBYDIGESTINGPBEWITHRESTRICTIVEENZYMEHINDIIXBAIANDASEIVECTORPEGFPCIISALSODIGESTEDBYASEIANDISLINKEDWITHBEFRAGMENTBYT4DNALIGASEAFTERITS5ENDISDEPHOSPHATASEDSOTHEMAMMARYGLANDSPECIFICEXPRESSIONALVECTORISCONSTRUCTEDWHICHCONTAINSREPORTGENEEGFPANTIBIOTICSGENEKANANEOREGULATORYELEMENTBOVINEBLGGENEUPSTREAMREGULATIONFRAGMENTANDFOREIGNTARGETGENECSFVE2GENE

簡介：EXPRESSIONSOFCLAUDIN1、ECADHERINANDTNF僅INCANINE艦～MMARYTUMOURSBYWANGFANGSUPERVISORPROFZHANGHAIBINATHESISSUBMITTEDTONANJINGAGRICULTURALUNIVERSITYINPARTIALFULFFILMENTOFTHEREQUIREMENTSF0RTHEMASTERDEGREEOFAGRONOMYCOMPLETEDINOCT2009COMMENCEMENTINDEC2009原創(chuàng)性聲明本人鄭重聲明所呈交的學(xué)位論文，是本人在導(dǎo)師的指導(dǎo)下，獨立進行研究工作所取得的成果。除文中已經(jīng)注明引用的內(nèi)容外，本論文不包含任何其它個人或集體已經(jīng)發(fā)表或撰寫過的作品成果。對本文的研究做出重要貢獻的個人和集體，均已在文中以明確方式標(biāo)明。本人完全意識到本聲明的法律結(jié)果由本人承擔(dān)。學(xué)位論文作者需親筆簽名盈％W唧年F胡，F(xiàn)日學(xué)位論文版權(quán)使用授權(quán)書本學(xué)位論文作者完全了解學(xué)校有關(guān)保留、使用學(xué)位論文的規(guī)定，同意學(xué)校保留并向國家有關(guān)部門或機構(gòu)送交論文的復(fù)印件和電子版，允許論文被查閱和借閱。本人授權(quán)南京農(nóng)業(yè)大學(xué)可以將本學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫進行檢索，可以采用影印、縮印或掃描等復(fù)制手段保存和匯編本學(xué)位論文。，保密口，在土年解密后適用本授權(quán)書。本學(xué)位論文屬于不保密講請在以上方框內(nèi)打“寸’學(xué)位論文作者需親筆簽名每％≯∞年I明，，日導(dǎo)師需親筆簽名矽1年F厶月，，日

簡介：EFFECTSOFGLUTAMINEONPROLIFERATIONANDHSP70EXPRESSIONOFMAMMARYEPITHELIALCELLSINDAIRYCOWSBYSHIHONGXIASUPERVISORPROFESSORWANGGENLINATHESISSUBMITTEDTONANJINGAGRICULTURALUNIVERSITYINPARTIALFULFILLMENTOFTHEREQUIREMENTSFORMASTERDEGREENANJINGAGRICULTURALUNIVERSITYNANJING，210095，PRCHINACOMPLETEDINNOVEMBER2009COMMENCEMENTINDECEMBER2009原創(chuàng)性聲明本人鄭重聲明所呈交的學(xué)位論文。是本人在導(dǎo)師的指導(dǎo)下，獨立進行研究工作所取得的成果。除文中已經(jīng)注明引用的內(nèi)容外，本論文不包含任何其他個人或集體已經(jīng)發(fā)表或撰寫過的作品成果。對本文的研究做出重要貢獻的個人和集體，均已在文中以明確方式標(biāo)明。本人完全意識到本聲明的法律結(jié)果由本人承擔(dān)。學(xué)位論文作者需親筆簽名生妞役，加聽年殷月／／日學(xué)位論文版權(quán)使用授權(quán)書本學(xué)位論文作者完全了解學(xué)校有關(guān)保留、使用學(xué)位論文的規(guī)定，同意學(xué)校保留并向國家有關(guān)部門或機構(gòu)送交論文的復(fù)印件和電子版，允許論文被查閱和借閱。本人授權(quán)南京農(nóng)業(yè)大學(xué)可以將本學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫進行檢索，可以采用影印、縮印或掃描等復(fù)制手段保存和匯編本學(xué)位論文。保密口，在一年解密后適用本授權(quán)書。本學(xué)位論文屬于不保密口。請在以上方框內(nèi)打“√’’學(xué)位論文作者需親筆簽名生乏L／嫠剔蒜勤獬陟卜劬侔皮月I多日勱叫年投其“B

簡介：大連理工大學(xué)博士學(xué)位論文抗奶牛乳腺炎特異性卵黃抗體的制備及活性研究姓名甄宇紅申請學(xué)位級別博士專業(yè)生物化工指導(dǎo)教師徐永平20081101抗奶牛乳腺炎特異性卵黃抗體的制備及活性研究凝塊得到明顯改善；體細胞數(shù)和細菌計數(shù)顯著降低。IGY和青霉素對實驗性乳腺炎的治愈率分別為83．3％和66．7％，對臨床型乳腺炎的治愈率分別為50％和33．3％。綜上所述，本研究成功制備出抗奶牛乳腺炎致病菌特異性IGY，考察了其體內(nèi)外活性，并證明了其對奶牛乳腺炎的療效優(yōu)于青霉素。為特異性IGY替代抗生素治療奶牛乳腺炎提供了依據(jù)。關(guān)鍵詞奶牛乳腺炎；卵黃免疫球蛋白；金黃色葡萄球菌；大腸桿菌

簡介：東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文紫杉醇致體外培養(yǎng)犬乳腺腫瘤細胞凋亡形態(tài)學(xué)及抗氧化功能影響的研究姓名任曉麗申請學(xué)位級別碩士專業(yè)臨床獸醫(yī)學(xué)指導(dǎo)教師劉云20090620ABSTRACTISTUDIESOFPACLITAXELONMORPHOLOGYOFAPOPTOSISANDEFFECTOFANTIOXIDEFUNCTIONOFCANINEBREASTTUMORCELLINVITROCULTUREABSTRACTAPOPTOSISWHICHOCCURREDINALLTHETUMORTISSUESWASCLOSELYRELATEDTOTHEOCCURRENCE,DEVELOPMENTANDMETASTASISOFTUMORCHEMOTHERAPEUTICDRUGACHIEVEDTHETHERAPEUTICEFFECTMAINLYBYINDUCINGAPOPTOSISOFCANCERCELLSTHEREFORE,INDUCINGAPOPTOSISOFTUMORCELLSBECAMEAHOTSPOTFORTUMORTREATMENTRESEARCHNATURALMEDICINESWHICHHADEXTENSIVESELECTIVITY,LITTLETOXICSIDEEFFECTANDLOWDRUGRESISTANCEWEREIMPORTANTTENDENCYOFSTUDYINGONANTITUMORWITHCHINESEMEDICINESPACLITAXELWASANEWNATURALBROADSPECTRUMANTITUMORDRUG,BYWHICHTHEMECHANISMOFAPOPTOSISINDUCEDWASTOLEADTOCELLCYCLEARRESTING2/MPHASEMAINLYBYRESTRAININGMITOSISOFCELLS,THUSTHENORMALMITOSISOFCELLSANDCELLMULTIPLICATIONWEREINTERRUPTEDCANINEBREASTTUMORWASAKINDOFTUMORDISEASEWHICHWASCLINICALLYCOMMONFORVETERINARYTHESTUDYOFEFFECTONCANINEBREASTTUMORWITHPACLITAXELHASN’TBEENREPORTEDYETHOMEANDABROAD,ANDDOGHADACLOSERELATIONWITHHUMAN,ALSOTHEREWASSIMILARASPECTINENVIRONMENTANDCAUSEOFCANCERBETWEENDOGANDHUMAN,SODOGSWEREUSEDASTUMORMODELANDSTUDIED,THEMECHANISMOFANTICANCEROFANIMALTUMORCELLSINDUCEDBYPACLITAXELWASAPPROACHED,WHICHPLAYEDAVERYIMPORTANTROLEINTREATINGANIMALTUMORANDTREATMENTSCHEMEFORHUMANCANCERTHISSUBJECTTOOKTHECANINEBREASTTUMORCELLSLINESCHMMSTRAINCULTUREDINVITROASAMODELTHECHMMWASTREATEDWITHDIFFERENTCONCENTRATIONSOFPACLITAXEL,CELLMULTIPLICATION,CYTOTOXICEFFECT,CHANGEOFCELLMORPHOLOGYANDANTIOXYGENFUNCTIONFACTORSUCHASSOD,CAT,MDAWEREDETECTEDAT24H,48HAND72HAFTERCULTURED,THETREATMENTTIMECONCENTRATIONEFFECTRELATIONSHIPWASANALYZEDANDTHECHANGEOFCELLMORPHOLOGYANDOXYFREERADICALSOFAPOPTOSISINDUCEDBYPACLITAXELWEREAPPROACHED,WHICHCOULDPROVIDEMORERELIABLEEXPERIMENTALDATAFORFURTHERSTUDYONTHEMECHANISMOFAPOPTOSISOFCANINEBREASTTUMORCELLSINDUCEDBYPACLITAXELASWELLASTHECLINICALAPPLICATIONOFPACLITAXELFORTHETREATMENTOFCANCERTHEEXPERIMENTALRESULTINDICATEDTHAT1CHANGEOFCANINEBREASTTUMORCELLSGROWTHEFFECTEDBYPACILITAXELWITHMTTCHROMATOMETRYANDTRYPANBLUEEXCLUSIONEXPERIMENTSWASDETECTED,THERESULTSINDICATEDINHIBITIONRADIOGRADUALLYINCREASEDWITHINCREASEOFPACLITAXELCONCENTRATIONANDCULTURETIME,BUTCELLSACTIVITYDECREASEDGRADUALLY,WHICHWASINTIMEDOSEDEPENDENTMANNERBYDETECTINGTHELACTICACIDDEHYDROGENASELDHACTIVITY,THERESULTSHOWEDTHATTHEREWERESIGNIFICANTDIFFERENCEBETWEENPACLITAXELGROUPSWITHDIFFERENTCONCENTRATIONSATTHESAMETIME（P005）,OTHERGROUPSHADSIGNIFICANT

簡介：關(guān)于學(xué)位論文原創(chuàng)性和使用授權(quán)的聲明本人所呈交的學(xué)位論文，是在導(dǎo)師指導(dǎo)下，獨立進行科學(xué)研究所取得的成果。對在論文研究期間給予指導(dǎo)、幫助和做出重要貢獻的個人或集體，均在文中明確說明。本聲明的法律責(zé)任由本人承擔(dān)。本人完全了解山東農(nóng)業(yè)大學(xué)有關(guān)保留和使用學(xué)位論文的規(guī)定，同意學(xué)校保留和按要求向國家有關(guān)部門或機構(gòu)送交論文紙質(zhì)本和電子版，允許論文被查閱和借閱。本人授權(quán)山東農(nóng)業(yè)大學(xué)可以將本學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫進行檢索，可以采用影印、縮印或其他復(fù)制手段保存論文和匯編本學(xué)位論文，同時授權(quán)中國科學(xué)技術(shù)信息研究所將本學(xué)位論文收錄到中國學(xué)位論文全文數(shù)據(jù)庫，并向社會公眾提供信息服務(wù)。保密論文在解密后應(yīng)遵守此規(guī)定。論文作者簽名瑚墜英文縮略詞乳腺炎‘葡萄球菌屬金黃色葡萄球菌鏈球菌屬停乳鏈球菌乳房鏈球菌大腸桿菌最小抑菌濃度加利福尼亞隱性乳腺炎檢測法JIIIIIRILITIILI,FLLLLLLIJ肌Y1786791。MASTITISGENUSSTRAPHYLOCOCCUSSTRAPHYLOCOCCUSAUREUSGENUSSTREPTOCOCCUSSTRAPHYLOCOCCUSDYSGALACTIAESTRAPHYLOCOCCTISUBEFISESCHEFI【CHIACOLIMICCMTCALIFOMIAMASTIFFSTEST