小鼠Sox2基因表達對P19干細胞向心肌細胞分化的影響.pdf

1、西北農(nóng)林科技大學碩士學位論文小鼠Sox2基因表達對P19干細胞向心肌細胞分化的影響姓名：雷蕾申請學位級別：碩士專業(yè)：臨床獸醫(yī)學指導教師：王華巖竇忠英20080501DOⅥNReGULATIONOFSOX2GENEEXPRESSIONAFFECTn叮GP19STEMCELLDIFFERENTIATIONINTOCARDIOMYOCYTESABSTRACT’ImemajnfktorsofexasperateheartRmctionaremy

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3、lar仃aJlsplaIltationtreatmentForthatreasons，Ⅱleresearchesfocusedonthemechallismofstemcendi彘rentiationi11t0cardiomyocytes，wllichhaveimportamsignificanceillt11eor)，andclimcP19ceⅡisae玎螂11icstemceIllinewmchpossessestllec印abil

4、i夠ofdi丘eremiationiIltocardiomyocyte111ecellulart啪splaIltation訛atmentⅡlatis嘲ectionor慨lspla11吼ion缸1ecardiacstemcellsbycorona巧renow、)l，：11ichareculturedinVi仃ototllepositionofnlyocardialiIlf打ctioncouldmakemyoc礬ialremodelingi

5、nt11eareaoflesionandiIIlproVet11e向nmionoffailinghI：artTheresearchstudies也ectl習mgeinmo印hologyandtIlec捌iospecificgeneexpression，wmchpr0Videaperfectcellularmodelforthe如ndaIllentalresearchesofcellular仃a11spl紐tation骶a_clllent

6、formyocardiali血幽0nThenomalP19cellsandthetreatedP19cellsⅥmose％婦geneⅥ郴血e疵redbyRNAiweres印蹦她1yinduceda11ddi髓fentiatedbyd曲etllylsulfoxide(DMSO)intocardiomyocytesThen，nleprocessofcenaggregationanddifrerentiationintocardiomyocy

7、tesw嬲testedDetectthechangeofcellsshape，thec砌iospeci丘cm出ere)【pression觚dexpressionofmRNAofcardiospeciflcgeIlesdllring吐ledid’erentiationintocardiomyocytesperiodbymo印hocytology，衄mlollistochemis缸yaIld弭PCRtestsn、vasshownthatce

8、llaggregatioIlSwerecoheringculturedwimgrow廿lcultu=remedium世erDMSOirlducedfor2days，spontaIleousanddlythmicallybeatingcellswerepresemwitllintheaggregationoutgrowtllson1OthdayOn4tlldaycellsappearedmsifo肌shape距donllthdaycell

9、swerearrayedparallellyconnect崦謝meachomernecellsshowedaaCtinpositiveandon5mdayexpressedc莉iomyocytespecificmRNAnlccardio—specialgeneswereeXpressedbyRTPCRandtheeXpression近creaSes謝m也einduciIlgallddi疵rentiationperiod，nle仃eate

10、dP19couldnotfomtheaggregations2ulddi虢rentiateint0cardiomyoc”ewimco—heringculturedTlliseXp商mentchoosemef魂ment證tllⅡlebestinte疵mgemciency鋤ausesiRNAintem血g刪geneItshow司nleiImuenceofstemcellsdi仃erentiationintocardiomyocyteswi廿