錢塘江三角魴胰島素樣生長因子-Ⅰ基因的克隆和表達研究.pdf

1、浙江大學(xué)碩士學(xué)位論文錢塘江三角魴胰島素樣生長因子Ⅰ基因的克隆和表達研究姓名：王群申請學(xué)位級別：碩士專業(yè)：基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師：童富淡20050501浙江大學(xué)碩士學(xué)位論文AbstractInsulinlikegrowthfactor1(insulin—likegrowthfactor1，IGFI)isaproteinthatinvolves70aminoacids，showingasmallmolecularweight7500DaIGF—

2、IbelongstothesuperfamilyofrelatedinsulinlikehormonewhichincludesIGF—I，IGFII，insulin，growthfactorandrelaxininvertebratesIGF—Ioffishservesasallmediatorforcelldivisiondifferentiation，embryonicdevelopment，growthregulation，ce

3、lldeathrestraintandosmosispressureregulationetc，andplaysallimportantroleinthegrowthandgenerationoffishes。Here，theinsulin—likegrowthfactorgeneofQiantangRiverTriangularBream(Megalobramaterminalis)wasclonedforthefirsttimefr

4、omliverbyRTPCRAndcloningvectorpMDl8—TIGF—1wasconstructedsuccessfullyThesequenceanalysisshowedtheQiantangRiverTriangularBreamIGF—IeDNAconsistedof486nucleotidesandencoded161aminoacidsincludingsignalpeptide，maturepeptide(B、

5、C、A、D)andEdomains，whichspannedthecompletesignalpeptide，matureIGF一1andEdomainSignalpeptidecontained132nucleotidesencoding44aminoacids；MatureIGFIcontained210nucleotidesencoding70aminoacidsandE—domainconsistedof141nucleotid

6、esencoding47aminoacidsAnalysisofEdomainindicatedthattheclonedQiantangRiverTriangularBreamIGFIbelongedtoIGFIEa一2subtype，Theinsulin—likegrowthfactorIgeneofQiantangRiverTriangularBreamwasaccessedGenBank(GenBankaccessionNoAY

7、2474121IdentityanalysisshowedtheIGF—Inucleotidesequenceshared998％homologywiththatofBluntnoseBream888％homologywiththatofGrassCarp，858％homologywiththatofCommonCarp；thepreIGF1aminoacidsequenceshared994％homologywiththatofBlu

8、ntnoseBream，888％homologywiththatofGrassCarp854％homologywiththatofCommonCarpIntheCyprinusCarpio，thehigherhomologyofnucleotidesequenceandaminoacidsequenceinIGF—IshowedthatthefisheshaveacloserrelationshipIGF—Igenewasclonedi