轉(zhuǎn)植酸酶基因膠凍樣芽孢桿菌（Bacillus mucilaginosus）NK-2的環(huán)境安全性及應(yīng)用效果研究.pdf

1、中國(guó)農(nóng)業(yè)科學(xué)院碩士學(xué)位論文轉(zhuǎn)植酸酶基因膠凍樣芽孢桿菌（Bacillusmucilaginosus）NK2的環(huán)境安全性及應(yīng)用效果研究姓名：李力申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：李俊20070601AbstractWiththerapidprogressofgeneongineexingtechnologyrrmreandrnofegeneticmodifiedorganismswemconstructedandusedinmicr

2、obialfertilizerBccanseoftheirlatentdangertoenvironmenttheevaluationfortheirbiosafetyisacrucialconsiderationbesidestheirefficiencyinyieldspromotionInthisarticle，thetransgenicswainNK2，withthecapabilityofdissolvingl【Pandsil

3、icatefrommineralsandproducingphytsseofAfumisatuswasevaluatedforitsbiosafetyinadditiontoyieldsincreasingperformancewithitsstartingstrainofDBt皿eresultsofthisresearchasfollows：1111epromotionefficiencyassessmentforyieldsbysq

4、uarefieldexperiment11Theresultsoffieldexperimentwithpotatorevealedth礬theⅡansgenicstrainNK2couldincreasethepotato’syieldssimilarlyasD4BIto247％withthedifferenceoverthesignificanttestinglevelTheresultsofthepotato’sphosphoru

5、s(P)contentsdemonstratedthatNK2couldsignificantlyincreasethePlevelt0946％withthePcontentof951ug／gcomparingwiththestartingswam2、1beresuItsoffieldexperimentwithtobaccoshowedthatNK2couldincreasethetobacco’syieldsto244％and171

6、％comparingwiththestartings劬lillandblankcontr01respectivelyTheresultsofthetobacco’sphosphorus口)contentsconfirmedthesuperiorityofNl【2withtheimprovomentof95％comparingwiththestartingstrainwiththedifferenceconformingthesignif

7、icanttesting2TheimpactofthetransgenicandstartingsHalnOllthequantityoftheculturablebacteriainsoilandtheirsurvivalinsoilbyenumeration01“1selectiveplate11Theperiodicalenumerationforthetotalculmrablebacteriainsoilbynutrientb

8、rothplateshowedthaLthedifferenceamongthethreetreatmentsamnotsignificantalthoughthetransgeniconesseemedhighestinthethreetreatments2111mperiodicalenumerationforthereleasedNK2andD4BIinsoilbyspecialselectiveplateshowedtIIa‘t

9、hedescendingtrendofthequantitiesofthetweleasedstrainsissame。andthemoffffdifiesare掣ea衙intheinitialpen00Afterthe57dthequantitiesoftherelease]strainsplungedsohugethattheirnumberswe∞almostundetectable3Theimpactofthethreetrea

10、tmentsonsoiln矗crobialcoilununitypropertieswe∞analyzedbyBIOI_DGECOMicroPlateformetaboficpotential，richnessandeveuuessofthesoilmicrobialcommunitiesbytheircarbonmetabolicprofiles1)theaveragewellcolordevelopment(AW∞)results，

11、usuallyregarded勰thesuggestionofmetabolicpotontialshowedthatthen己atnEntofNK2couldemhanenthemetabolicpotential80greatthattheAWCDincreased188％thanthestartingstrainand353％thanblankcontr01impliedthesuperiorperformanceOilyield

12、spromotionwascloselyrelatedwiththehighestmetabolicpotential2)theanalysisfortheirrichnessandcvellflessbyShannonWeinnerrichnessindex(II’)andShannonWeinnerevennessindex(E)ofthethreesoilmicrobialcommunitiesshowedthatwitllthe