VDR基因多態(tài)性與兒童喘息性疾病的相關性研究.pdf

1、內(nèi)蒙古醫(yī)學院碩士學位論文VDR基因多態(tài)性與兒童喘息性疾病的相關性研究姓名：王琳申請學位級別：碩士專業(yè)：兒科學指導教師：甄艷芬管京生20090501PolymorphismsofVitaminDReceptorGeneandItsAssociationwithChildrenAsthmaticDiseasesAbstract，Objectivemoughstud再ngtheassociationoftheVitaminDreceptorg

2、enepolymorphismswithchildrenasthmaticdiseases，thispaperexploresthehereditarysusceptibilityfac—tomofchildrenasthmaandasthmaticbronchitisMethodsThispaperappliesthemethodsofpolymerasechainreaction—restrictionfragmentlengthp

3、olymorphism(PCR—RFLP)andgeneticanalysis，totestVDRgeneApaI—polymorphismforassociationwithasthnlariska_mong30Childrenwithasthmas，40childrenwithasthmaticbronchitisandthecontrolgroup‘composedof40normalchildrenResultsSignific

4、antdifferences眥foundinthefrequencydistributionofVDRgenotypesamongthreegroups(X2=11726，P=O020)TherearesignificantdifferencesinthefrequencydistributionofVDRgenotypesbothinthegroupsofasthrnaandthenormal(X2=9317，P=o009)，asth

5、maticbronchitis(x2=10546，P=o005)Asignificantdifferenceisn’tfoundbetweenthegroupsofasthmaticbronchitisandthecontrol(X2=o06，P=o97)Significantdifferences眥foundinVDRallelesfrequenciesofApaIpolymorphismamongthreegroups(x2=856

6、1，P=0014)Therea弛significantdifferencesinVDRallelesfrequenciesofApaIpolymorphismbothinthegroupsofasthmaandthenormal(x2=6850，P=O009)，asthmaticbronchitis(X2=7622，P=O006)Asignificantdifferenceisn’tfoundbetweenthegroupsofasth

7、maticbronchitisandthecontrolgroups(X2=0028，P=0867)Conclusion(1)ThedistributionofApaIpolymorphismofthechildrenVDRgeneinHohhotdistrictisbalanced，andtheaagenotypeaccountedfor50％Thefrequencyofaalleleis675％(2)Thereistheassoci

8、ationbetweenApalpolymorphismoftheVDRgeneandasthmaThisstudyraisesthepossibilitythatVDRgenepolymorphismmightbeimportantinanindividualssusceptibilitytodeveloptheasthma(3)Thereisn’ttheassociationbetweenApaIpolymorphismoftheV