三氧化二砷對(duì)A375人惡性黑素瘤細(xì)胞株黑素生成和細(xì)胞凋亡的研究.pdf

1、遵義醫(yī)學(xué)院碩士學(xué)位論文三氧化二砷對(duì)A375人惡性黑素瘤細(xì)胞株黑素生成和細(xì)胞凋亡的研究姓名：肖和申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：皮膚性病學(xué)指導(dǎo)教師：張信江李大鐵20040301畢業(yè)論文三氧化二砷對(duì)A375人惡性黑素瘤細(xì)胞株黑素生成和細(xì)胞凋亡的研究論文Effectsofarsenictrioxideonthecellproliferation、tyrosinaseactivity、melanogenesisandcellapoptosisinthe

2、humanmalignantmelanomacelllinesA375AbstractObjectivesTostudytheeffectsofmultiandsingleprescriptionofarsenictrioxide(As203)onthecellproliferation、tyrosinaseactivityandme|anogenesisinA375humanmalignantmelanomacellsAndtoinv

3、estigatetheapoptosistrigeringeffectofarsenictrioxideonthehumanmalignantmelanomacelllinesA375MethodsMTTreductionassayandmorphotogicchangeswereusedteflecttheproliferationofA375humanmalignantmelanomacells；Measuresofthetyros

4、inaseactivityandthemelanincontentwereusedtostudythechangesoftyrosinaseactivityandthemelanincontentofA375cellsbydetectionofenzymeThemorphologicalchangesandapoptoticcharacteristicoftheA375cellswereobservedbytransmissionele

5、ctronmicroscopeTheapoptosisrateandthechangesofcellcycleweredetectedbyflowcytometryResultswhentheconcentrationofarsenictrioxideislowerthanO019mol／L，anactiveeffectshowed，suchascellsproliferation、tyrosinaseactivityascending

6、、melanogenesisincreasingAndaninhibitoryeffctappeared，whentheconcentrationofarsenictrioxideisabovetheO01pmol／L，evencytotoxicactionabovethe109mol／LFrom1OItmol／Lto40Itmol／L，apoptoficbodyofA375celllineswasobservedbytransmiss

7、ionelectronmicroscopeA375celllinestreatedwith20I_tmol／Land409mol／LAs203for72hoursshowedatypicalhypodiploidpeakbeforeOlphaseatthesametime，theA375celllineswereseriouslyblockedattheSphase。themaximumwas5485percentTreated麗tll