順鉑白芨混合微球誘導大鼠肝癌細胞凋亡的實驗研究.pdf

1、Y957932學院代號專業(yè)代碼學號密級陜西中醫(yī)學院ShaanxiUniversityofChineseMedicine碩士學位論文順鉑白芨混合微球誘導大鼠肝癌細胞凋亡的實驗研究學位申請人專業(yè)名稱申請學位類型李紅曉中西醫(yī)結(jié)合臨床外科專業(yè)學位指導教師姓名王治全教授主任醫(yī)師論文提交19期2006年4月TheresearchontheapoptosisofhepatomaoeIIsinducedwithcispIatinsbIetiIIamix

2、edmiorospheresAbstractIObjeetivel：Toinvestigatetheeffectsandmechanismofmixedmicrospheresontheapoptosisinhepatomacells，andexplorethevalueofitsapoptosisinducedbymixedcisplatinsandbletillamicrospheres【Methods]：fortymousewer

3、erandomlydividedintofourgroups(controlgroup，mixedbletiUamicrospheresgroup125][group，cisplatinsgroup)，and16foreachgroupChromationcondensationwasobservedbyCOstainingwithfluorescentdyesApoptotissubG1wasdetectedbyflowcytomet

4、ryandDNAladderWaSobservedusingagarosegelelectrophoresisIResultsl：BletillaincombinationwithcisplatinWasfoundtohave3ynergeticeffectsontheproliferationofhepatomacellsnledatashowedthathepatomacellstreated謝t11bletillaandcispl

5、atinincombinationproducedinternucleosomalDNAflagmentationanalysedbyagarosexlactrophoresisTl倫resultsofflowcytometryshowedthatbletillaandcisplatinadministratedincombinationshowednoobviouschangeinG1phasedistributioncompared

6、withsingletreatmentHoweverthiscombinationreducedtheSphasearrestandreversedthereductionofG2／MphaseinducedbycisplatintreatmentTheresultsalSOshowedthatthereWas113％or937％ofcellsundergoingapoptoticcellsinhepatomacellstreatedb

7、ycisplatinrespectivelywhileitshowed324％ofapoptoticcellsincombinationtreatmentCisplatin“3IandmixedmicrospheresWaSshowntoinducetypicalc11romatincondensationinhepatomacells【Conclusion]：Theresultssuggestthatbletillaenhanceme

8、ntofcisplatininducedapoptosisassociateswithdecreaseofproteinexpressionwhichmaybeUsefulforcanceerchemotherapy【Kcywords]：cisplatinsbletillamixedmicrospheres(CBMM)；cellapotosis；hepatoma；CI’splatinsAuthor：LihongxiaoTutor：Wan