消癥丸對(duì)膠質(zhì)瘤C6細(xì)胞增殖抑制和誘導(dǎo)凋亡及Bcl-2表達(dá)影響的實(shí)驗(yàn)研究.pdf

1、X777837學(xué)院代號(hào)專業(yè)代碼學(xué)號(hào)!!!!!!塑密級(jí)——陜西中醫(yī)學(xué)院ShaanxiUniversityofChineseMedicine碩士學(xué)位論文消癮丸對(duì)膠質(zhì)瘤C6細(xì)胞增殖抑制和誘導(dǎo)凋亡及Bcl一2表達(dá)影響的實(shí)驗(yàn)研究學(xué)位申請(qǐng)人李淑芳專業(yè)名稱中西醫(yī)結(jié)合臨床(腦病)申請(qǐng)學(xué)位類型臨床醫(yī)學(xué)碩士專業(yè)學(xué)位指導(dǎo)教師姓名協(xié)助指導(dǎo)教師陳美芳教授閏詠梅教授論文提交日期2005年4月nhibitionofproIiferationandinductiono

2、fapopotosisofC6gIiomaceJIsbyXiaozhengWanwithMedicatedSerumABSTRACTOjective：ToinvestigateXiaozhengWan(XZW)withMedicatedSerumontheproliferationandinducingtheapopotosisofC6gliomacellsMethods：UsingserumpharⅢacologicmethod，th

3、eC6cellswasobservedbyco—culturingwithdifferentdosagesofmedicatedserumcontainingXZWMTTwasusedtomeasurethelevelsoftheproliferationofC6culturedwithmedicatedserumcontainingXZWindifferentdosageandtimepointsTheinduceapopototic

4、ratewasobservedbyflowcytometry(FCM)ThemorphologicalchangeofC6cellswasdetectedbyelectronicmicroscopyImmunohistochemistrywasusedtoexaminetheexpressionofBcl一2aftertreatmentofmedicatedserumcontainingXZWResults：Thedifferentdo

5、sagesofmedicatedserumcontainingXZWcouldesignificantlyinhibittheproliferationofC6cells，andinhibitoryraterevealedamedicatedserumdose—timedependentmannerTheoutcomeofFCMshouldthattheapopototiccellratewasinadosedependentmanne

6、rThemorphologicalchangeofapopotosiswerealsofoundbyelectronicmicroscopyXZWdecreasedexpressionofBcl一2intheC6cellsConclusion：XZWinhibittheproliferationofC6，whichwasassociatedwithinhibitingtheexpressionofBcl一2，andinducetheap