我國(guó)不同地域牦牛β-乳球蛋白5′調(diào)控區(qū)同源性的分析.pdf

1、武漢科技大學(xué)碩士學(xué)位論文我國(guó)不同地域牦牛β乳球蛋白5′調(diào)控區(qū)同源性的分析姓名：朱艷君申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：環(huán)境工程指導(dǎo)教師：劉建忠20051115第1I頁(yè)武漢科技大學(xué)碩士學(xué)位論文AbstractGeneticpolymorphismisanimportantpartofbiologicaldiversityandisthebasisofthediversityofecosystem，speicesdiversityandmainten

2、ancethespeciestosurviveoveralongperiodInthisstudyapair9fprimersforPCRweredesignedaccordingteferencesp曲lished，the，oligonucleotideswereasfollow：theupstreamprimer5gCg，AAT，TCA，TCC，CAC，gTg，CCTgC3’，downstreamprimer5’gag，從TTCC，

3、TggggA，ggg，ACC，11：3’，afI母30cyclesofPCRprocedureswithannealingtemperatureat68“C，afragmentof1447bpWasamplifiedandWaScollectedfromagarosegelThenthefragmentWasclonedintopMDl8Tvectorandsequenced，nesequencingresultsindicatedth

4、attherewere34nucleotidesmutationinthe5regulatoryregionoflactoglobulingenebetweendairyCOWandQinghaiyakandthehomologoussequencesWaS9765％，theeminentdifferenceswerethattherewasacontinuousdeletemutationofthreenucleotidesatone

5、siteandoneinsertmutationofasinglenucleotideatanothersiteinQinghai，yak’SgenomeTherewere38nucleotidesmutationinthe5’regulatoryregionoflactoglobulingenebetweendairyCOWandYunnanyakthehomologyWas973％Theeminentdifferenceswerea

6、lSOacontinuousdeletemutationofthreenucleotidesatonesiteandoneinsertmutationofasinglenucleotideatanothersiteinYunnanyak’SgenomeTherewere8nucleotidesmutationinthe5’regulatoryregionoflactoglobulingenebetweenQinghaiandYunnan