17164.幾種新型熒光探針的細(xì)胞毒性研究及其在成像分析中的應(yīng)用

1、分類號學(xué)校代碼10542密級學(xué)號2Q120212104l幾種新型熒光探針的細(xì)胞毒性研究及其在成像分析中的應(yīng)用Thecytotoxicityofseveralfluorescentprobesanditsapplicationsinimaging研究生姓名彌娜修指導(dǎo)教師姓名、職稱張友玉教授學(xué)科專業(yè)藥物分析學(xué)研究方向生化分析與生物傳感湖南師范大學(xué)學(xué)位評定委員會辦公室二零一五年四月ABSTRACTFluorescenceimagingtech

2、nologyhasgoodselectivityandhighsensitivityInrecentyears，ithasattractedmoreandmoreattentionandhasbeenwidelyusedinvariousfieldsofbiological，environmental，medicalandothersBecauseofthefluorescentstabilityandlowtoxicity，molec

3、ularfluorescentprobesandfluorescentnano—materialsareusedforbiologicalimagingTherefore，wesynthesizedseveralfluorescentprobestostudytheirapplicationsinbiologicalanalysisThemain’contentsweresummarizedasfollows：(1)Inthiswork

4、，anovelfluorescentprobewithhighselectivityhasbeendevelopedforlivecellimagingofcysteine(Cys)TheprobeisdemonstratedtohaveexcellentbiocompatibilityhighintracellulardeliveryefficiencyandstabilityCellcytotoxicityandbioimaging

5、studiesrevealedthatprobewascellpermeableandcouldbeusedtodetectCysinlivingcellwithlowcytotoxicity(2)Anovelfluorescentprobewithhighselectivityhasbeendevelopedforlivecellimagingofcysteine(Cys)，homocysteine(Hcy)andGlutathion

6、e(GSH)TheprobeisdemonstratedtohaveexcellentbiocompatibilityhighintracellulardeliveryefficiencyandstabilityforobservationsCellcytotoxicityandbio—imagingstudiesrevealedthatprobewascellpermeableandcouldbeusedtodetectCyswith