體外誘導培養(yǎng)雞胚破骨細胞及鈣、磷對其活性的影響.pdf

1、揚州大學碩士學位論文體外誘導培養(yǎng)雞胚破骨細胞及鈣、磷對其活性的影響姓名：薛永峰申請學位級別：碩士專業(yè)：動物營養(yǎng)與飼料科學指導教師：章世元201005012揚州人學碩I：學位論文Osteoclastsfromthechickembryoinduceculturedby1，25一DihydroxyvitaminD3andtheeffectsofllciumphosphorusontheactivityofthe●steoclastscalc

2、iumOnUleactlVloItlleosteoclasts，Masterpost—graduate：XueYongFengAdvisor：AssociateProfessorZHANGShiyuan(CollegeofAnimalScienceandTechnologyYangzhouUniversityYangzhou225009，China)AbstractThisexperimentwaseonductedtoresearch

3、therelationshipbetweentheformationandactivationofosteoclastsandbonemetabolismofthepoultryinthecellularandmolecularlevelsOnthebasisofinduceculturedosteoclastsofchickembryo，theformandfunctionoftheseOCwereobservedandidentif

4、iedbyphasecon觚tmicroscope，histochemicalstainingfortartrateresistantacidphosphatase(TRAP)stainingatthefirstandtheeveryotherday，anddetectionofboneresorptionpitsthroughscanningelectronmicroscopyattheseventhdayTostudytheimpa

5、ctonosteoclastsformation，activationandthefunctionofboneresorptionforthefactorsofcalciumandphosphorus，andprovidethescientifictheoryforresearchingthebonemetabolismdiseaseofpoultryThetestresultsareasfollows：(1)Theosteoclats

6、ofthechickembryowereculturedbyusing125DhydroxyvitaminD3inductionSpecificityofosteoclastsWasidentifiedbythemeansthatmentionedaboveTheresultsprovedthattheinducedosteoclastshadtheactivityofmatureosteclasts，andthebestinduced

7、concentrationof125一DhydroxyvitaminD3was10—6mol／L；(2)Withouttheinductionofl，25DhydroxyvitaminD3，andadddifferentconcentrationsofcalcium，phosphorustothetestgroups，wefoundthathighconcentrationsofcalcium，phosphoruscouldinhibi

8、ttheofviabilityosteoclatsinshorttime(16h)，whilemiddleconcentrationgroupsandlowconcentrationgroupsinhibitedtheofviabilityosteoelats24hourslaterTo32hours，thedifferencebetweenhi【ghconcentrationgroupsandmiddleconcentrationgr

9、oupsWasnotsignificantbutlowerconcentrationsweresignificantlydifferentfrommiddleconcentrationgroups，andthedifferencebetweeneachtestgroupandcontrolgroupWassignificant；(3)Theresultsofaddingdifferentconcentrationsofcalciuman