雌激素及雌激素α受體PvuⅡ和XbaⅠ基因多態(tài)性與宮頸癌關(guān)系的研究.pdf

1、山西醫(yī)科大學(xué)碩士學(xué)位論文雌激素及雌激素α受體PvuⅡ和XbaⅠ基因多態(tài)性與宮頸癌關(guān)系的研究姓名：王志敏申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：流行病與衛(wèi)生統(tǒng)計(jì)學(xué)指導(dǎo)教師：王金桃20070426山西醫(yī)科大學(xué)碩士學(xué)位論文Abstractobjective：Developmentofcervicalcancerisassociatedwithmanyfactors，amongthem，geneticbackgroundplayasignificantrole

2、SeveralreportsindicatethatestrogenreceptoragenepolymorphismmighthavearelationwithmanykindsoftulnorsusceptibilityHowevertherehavenostudiesaboutthereportbetweenestrogenreceptoragenepolymorphismandcervicalCancerInthisstudyw

3、ewouldmakeapenetratedapproachoftherelationbetweenestrogen，ERaPvuII和XbaIgenepolymorphismandcervicalcancerMethod：Ahospitalbasedeasecontrolstudywasconducted102caseswithcervicalcancerand125controlswithgynecologybenigntumorby

4、pathologicaldiagnosedfromShanxiTumorHospitalwereinvolvedinthisstudyfromSeptember2004toSeptember2005AllparticipantswareHannationalityandhadinhabitedinShanxiprovinceformorethantwentyyearsThepregnant，previousandpresentovari

5、espatients，otherCanCarpatients，steroidhormoneusersorpsychoticpatientswereexcludedAllcasesandcontrolsWCl“einvestigateddirectlybymedicalstaffwiththe$anlequestionnairesThequestionnairesincludedbasicinformation，educationando

6、thersocioeconomicfactors；generallifestylehabitssuchassmokingandalcoholconsulEIption；sexualbehaviorandheathhabits；menstrual，marriageandpregnancyhistory；medicalhistoryandtumorfamilyhistoryPeripheralbloodsamplewerecollected

7、fTomallparticipantsinNO8～10dayofmenstrualcycleEstradiolwasdetectedbyELlSAassaysERaPⅦIIandXbaIgenepolymorphismwetedeterminedbyPCRRFLPmethodAlldatawareanalyzedbySPSSl30，usingftestttest，univariateandmultiplenonconditionallo

8、gisticregressionanalysistesttheassociationbetweenthelevelofE2，ERaPvuIIandXbaIgenetype，correlationfactorsandcervicalCanCarResult：(1)Theresultofsamplefactoranalysisshowedthatculture，occupationsmoking，stateofmenses，ageatfir

9、stsexualintercourse，ageatfirstpregnancyageatfirstprocreate，timesofpregnancyandtimesofpantywereallassociated謝thcervicalcancer；(2)Estradiolofcase(4757_|2427pg／m1)wasmorehigherthancontrol(4757：￡2427pg／m1)withstatisticsignif

10、icancebetweentwoarrays；(3)TheanalysisofinteractionbetweenthestateofmensesandestradiolleveltocervicalCanC豇indicatetherewasinteractionbetweentwofactors；(4)ThedistributionofERaPvuIIandXbaIgenepolymorphismconsistentwithHardy

11、—Weinbergbalance；(5)ComparingthefrequenciesofhomozygosismutationtypeXbaIXXgenotype，heterozygosismutationtypeXxgenotypetowildtypexxgenotypeofca∞andcontr01thedifferencebetweenXbaIXXgenotypeandXbaIxxgenotypehadnostatisticsi

12、gnificance(f=0573，P=0449)，butthereWasstatisticsignificancebetweenXbaIXxgenotypeandXhaIXXgenotype(f=4372，P=o037)；mergingthefrequenciesofXbaIXXandXxgenotype，comparingwithXbaI)【)【genotypeandthedifferencehadstatisticssignifi