31246.sertoli細胞吞噬凋亡生精細胞的機理和意義

1、北京協(xié)和醫(yī)學院中國醫(yī)學科學院博士學位論文Sertoli細胞吞噬凋亡生精細胞的機理和意義姓名：熊偉鵬申請學位級別：博士專業(yè)：細胞生物學指導教師：韓代書陳實平20090501北京協(xié)和醫(yī)學院博上研究生學位論文AbstractAbout75％ofspermatogeniccellsareestimatedtoundergoapoptosisduringmammalianspermatogenesis，andthecytoplasmicporti

2、onsoftherestgermcellsareshedandformresidualbodiesbeforetheymatureintospermatozoaTheapoptoticspermatogeniccellsandresidualbodiesarephagocytosedanddegradedbySertolicellsThemechanismsandsignificanceunderlyingthesephenomenar

3、emaintobeclarifiedInthisstudyweinvestigatetheseissueswithaneffortontheessentialroleofTyr03receptortyrosinekinasesubfamilyinregulmingphagocyticabilityofSertolicells，andthemeaningofphagocytosisofapoptoticgermcellbySertolic

4、ellsWeestablishedaninvitromethodtoevaluatethephagocyticabilityofSertolicellsthroughdetectinglipiddropletformationByusingthismodel，weobservedt11atTyr03subfamilyofreceptortyrosinekinases(RTKs)——Tyr03，Axl，MerandtheirligandG

5、as6regulatephagocyticabilityofSertolicellsThepresenceofGas6inserumfreemediumincreasedphagocyticabilityofSertolicellsby5timesByanalysisoftheTyr03RTKsdeficientSertolicells，wedemonstratedthatthethreemembersofTyr03RTKsactcoo

6、perativelyinregulatingthephagocyticabilityofSertolicellsMerisresponsiblefortriggeringphagocytosisofapoptoticspermatogeniccellsbySertolicells，Tyr03andAxlenhancecooperativelytheactionofMerbyrecognizingandbindingapoptoticce

7、llsFurthermore，wedemonstratedthatapoptoticspermatogeniccellsandresidualbodiesCanbeenergysourcesforSertolicells／nvitroassayshowedthatengulfmentofapoptoticspermatogeniccellsincreasesATPproductionbySertolicellsTheATPproduct

8、ioninseminiferoustubulesiscloselyrelatedtothephagocytosisofresidualbodiesbySertolicellsInducedapoptosisofspermatogeniccellsinvivoincreasedATPlevelinseminiferoustubulesTheATPproductionbothinvivoandinvitroassociatedwiththe

9、lipidformationinSertolicellsafterphagocytosisofapoptoticspermatogeniccellsWethereforeconcludethatafterphagocytosisbySertolicells，apoptoticspermatogeniccellsaredegradedtoformlipids，whichcanbeusedtoproduceATPTheresultsdefi

10、neanovelmeaningofspermatogeniccelldeathFinally，wefoundthatlysatesofapoptoticgermcellsCantriggerinflammatoryresponsesbySertolicellsThelysatesinducedexpressionofvariousinflammatorycytokinesinSertolicellsThisprocessdependso