中國對蝦血細胞單克隆抗體的研制及其應用.pdf

1、青島海洋大學碩士學位論文中國對蝦血細胞單克隆抗體的研制及其應用姓名：張利峰申請學位級別：碩士專業(yè)：水產養(yǎng)殖指導教師：戰(zhàn)文斌周麗2001.6.1青島海洋犬掌水產掌院碩‘論文2001——————●——————_——_———_——————_——、●———H——●—_———_^—_—●———_——_——●—————————●—●—●——————————————————一一AbstractInordertostudyimmunecellulare

2、ffectorsinshrimp(Penaeuschinensis)，threetypesMcAbswereproductedbyimmunisingBalb／cmousewithhaemocytesthatcarlidentifythreehaemocytesubpopulationsofPenaeuschinensis，andusingthoserevealedtheimmunecharacterizationofreceptorl

3、ocatedonthethreehaemocytesubpopulationsofPenaeuschinensis，themainmeansasfellow：Inthisstudy，Balb／cmicewereimmunizedwiththehaemocytesofshrimp，polyethyleneglycol(PEG)wasusedassolutionforcellfusion，andhybridomaswasselectedby

4、meansofenzyme—linkedimmunosorbentassays(ELISA)andImmunofluorescenceassays(IrA)Twenty—threestrainsofhybridomaswhichproducemonoclonalantibodiesagainsthaemocytesofshrimpTherearethirteenstrainhi【曲一affinityMcAbswereselectedby

5、IFA，andusetheMcAbscanidentifythreetypeshaemocytesofshrimp，3A3、3A2、3A5、3A6、382and585McAbsshowedspecificallyreactivitywithhyalinecellandrecognisedthereceptorlocatedonthesurfaceofhyalinecelli383、3C2、3C4、5A2and582McAbsspecif

6、icallyboundtosmallgranularcellwhilerecognisingthecellmembrancereceptorlocatedonthesurfaceandcytoplasmreceptorofsmallgranularcell386、4A4McAbsreactedonlywithlargegranularcellandwereabletecognisethecellmembranereceptorwhich

7、locatedonthesurfaceoflargegranularcellInthisstudyusingtheproducedMcAbstevealthegenesisanddistributionofhaemocyteinpostlarve(Penaeuschinensis)，theresultsisthattherearemanyofhyalinecellsintheantenna，thegatheringcellsindica